Table 7.

Volume and working concentrations of reagents used to differentiate NES cells and modulate redox homeostasis

NES differentiation medium+ antioxidant	Volume for 50 mL	Concentration
DMEM/F-12+GlutaMAX	49 mL	-
N2	500 μL	1:100
B27/B27-MA	500 μL	1:100
P/S	500 μL	1:100 (optional)
NAC	40.85 μL (0.5 mM) or 204.25 μL (2.5 mM)	1:1224 (0.5 mM) or 1:244.8 (2.5 mM) of a 0.612 M stock

Neural induction medium 1 and 2 should be prepared fresh every day. NES growth medium, NES differentiation medium and NES differentiation medium + antioxidants can be stored at 4°C for 1 week.