REAGENT or RESOURCE	SOURCE	IDENTIFIER
Antibodies
Rabbit monoclonal anti-β-tubulin III Working dilution, 1:1000	Merck	Cat T2200. RRID:AB_262133
Rabbit monoclonal anti-SOX2 (D6D9) Working dilution, 1:1000	Cell Signaling	Cat #3579 RRID:AB_2195767
Mouse monoclonal anti-Nestin Working dilution, 1:500	Millipore	MAB5326 RRID:AB_11211837
Chemicals, peptides, and recombinant proteins
DMEM/F-12, GlutaMAXTM supplement	Thermo Fisher Scientific	31331-028
N-2 Supplement (100×)	Thermo Fisher Scientific	17502-048
B27TM Supplement (50×), Serum free	Thermo Fisher Scientific	17504-044
B27TM Supplement minus antioxidants (B27-MA ; 50×) Serum free	Thermo Fisher Scientific	10889038
Poly-L-ornithine hydrobromide	Merck	P3665. CAS Number: 27378-49-0
Laminin from Engelbreth-Holm-Swarm murine sarcoma basement membrane	Merck	L2020. CAS Number: 114956-81-9
Soybean Trypsin Inhibitor, powder	Thermo Fisher Scientific	17075-029
Human bFGF Recombinant Protein	Thermo Fisher Scientific	13256-029
Animal-Free Recombinant Human EGF	PeproTech	AF-100-15 Accession number: P01133
N-Acetyl-L-cysteine	Merck	A9165 CAS Number: 616-91-1
Essential 8 Medium Kit (E8) (Basal medium and supplement; 50×)	Thermo Fisher Scientific	A1517001
ROCK Inhibitor (Y-27632)	Merck	SCM075
Human recombinant laminin 521	BioLamina	LN521-02
KnockOut™ Serum Replacement	Thermo Fisher Scientific	10828028
Non-essential Amino Acids 100×	Thermo Fisher Scientific	11140035
2-Mercaptoethanol 50 mM	Thermo Fisher Scientific	31350010
Penicillin-Streptomycin 10,000 U/mL	Thermo Fisher Scientific	15140122
Neurobasal™ Medium	Thermo Fisher Scientific	21103049
TrypLE™ Select Enzyme (1×)	Thermo Fisher Scientific	12563011
Recombinant Human Noggin	PeproTech	120-10C
SB431542	Merck	S4317-5MG
CHIR99021	Merck	SML1046-5MG
DPBS, no calcium, no magnesium	Thermo Fisher Scientific	14190169
BSA	Merck	A7030
GlutaMAX™ Supplement	Thermo Fisher Scientific	35050061
Experimental models: cell lines
Human neuroepithelial stem cells	Falk Lab	N/A
Induced pluripotent stem cells	Falk lab	N/A
Critical commercial assays
Lonza P3 Primary Cell 4D-Nucleofector Kit S or L	Lonza (Fisher Scientific)	V4XP-3024 or -3032 (Lonza) 13429329 or 13439329 (Fisher Scientific)
Other
Plastic dishes	Sarstedt	N/A
Cryo vials	VWR	479-1261P
12-Well plates	VWR	734-2324
4D-Nucleofector	Lonza	AAF-1002B