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. Author manuscript; available in PMC: 2021 Nov 1.
Published in final edited form as: Nature. 2021 Apr 14;593(7858):294–298. doi: 10.1038/s41586-021-03458-7

Extended Data Fig. 10. Both LR and SR synaptic complexes are able to perform double ligation during NHEJ in vitro.

Extended Data Fig. 10.

a, Substrate design for the ligation assay. An Internal Cy5 label is added to only the right half of the substrate to visualize the ligation products. A 4 nt 3’ complementary overhang has been introduced on both sides of the substrate. b, Denaturing gel analysis of end-joining by the LR complex. 100nM of DNA, 200 nM of DNA-PKcs, and Ku70/80, 500nM of XLF, and 70nM of X4L4 were added, respectively. Asterisk indicates alternative secondary structure or impurity of the cy5-labeled oligo. Size of the DNA substrates and ligation products are labeled on the left (unit: bp) c, Denaturing gel analysis of end-joining by the SR complex. The final factor concentrations are the same as in b. For gel source data, see Supplementary Figure 1. Similar conditions for either of the gel have been replicated as biological replicates for two times.