Skip to main content
. Author manuscript; available in PMC: 2022 May 13.
Published in final edited form as: Cell. 2021 Apr 28;184(10):2779–2792.e18. doi: 10.1016/j.cell.2021.03.043

Figure 2. PsychLight enables the detection of endogenous serotonin dynamics during fear conditioning using fiber photometry.

Figure 2.

A. Expression of psychLight2 in the DRN, BNST, BLA and OFC near the location of fiber implantation. Scale bars: 500 μm B. Schematic illustrating the design of auditory fear conditioning experiments (30 s tone co-terminating with a 1.5 s foot shock, n = 15 presentations). C–F. Single-trial heatmap and averaged-trial traces of serotonin dynamics in DRN (C, n = 135 trials from 9 animals), BNST (D, n = 120 trials from 8 animals), BLA (E, n = 90 trials from 6 animals), and OFC (F, n = 90 trials from 6 animals) in response to a tone (blue) and foot shock (pink). ROC plots indicate true detection rate (TDR) against false positive rate (FPR), and d′ is calculated by avg(z-scoreshock)/std(z-scorebaseyne). Average traces indicated by solid lines; shaded area represents S.E.M. DRN = dorsal raphe nucleus; BNST = bed nucleus of the stria terminalis; BLA = basolateral amygdala; OFC = orbitofrontal cortex. See also Supplementary Fig. 2E–F.