| Bacterial and Virus Strains |
| NEB 5-ALPHA COMPETENT E.COLI (HIGH EFFICIENCY) - 6X0.2 ML |
NEB |
C2987I |
| rAAV_hSyn_psychLight2 |
Vigene |
N/A |
| Chemicals, Peptides, and Recombinant Proteins |
| lysergic acid diethylamide hemitartrate |
NIH Drug Supply Program |
N/A |
| psilocin |
NIH Drug Supply Program |
N/A |
| psilocybin |
NIH Drug Supply Program |
N/A |
| 2-(4-Iodo-2,5-dimethoxyphenyl)ethan-1-amine hydrochloride (2C-I) |
NIH Drug Supply Program |
N/A |
| 2-bromo-lysergic acid diethylamide tartrate (BOL-148) |
NIH Drug Supply Program |
N/A |
| ibogaine hydrochloride |
NIH Drug Supply Program |
N/A |
| noribogaine |
NIH Drug Supply Program |
N/A |
| cocaine hydrochloride |
NIH Drug Supply Program |
N/A |
| salvinorin A |
NIH Drug Supply Program |
N/A |
| phencyclidine hydrochloride (PCP) |
NIH Drug Supply Program |
N/A |
| serotonin hydrochloride (5-HT) |
Fisher |
50–120–7920 |
| ketanserin (KETSN) |
ApexBio |
50–190–5332 |
| ketamine hydrochloride (KET) |
Fagron |
803647 |
| morphine sulfate |
Mallinckrodt, Inc. |
0406–1521–53 |
| lisuride maleate (LIS) |
Tocris |
40–5210 |
| bromocriptine mesylate |
Tocris |
04–275–0 |
| (±)-2,5-dimethoxy-4-iodoamphetamine hydrochloride (DOI) |
Cayman |
13885 |
| imipramine hydrochloride |
Cayman |
15890 |
| modafinil |
Cayman |
15417 |
| (±)-threo-methylphenidate hydrochloride |
Cayman |
11639 |
| indole 3-aceitic acid (3-IAA) |
ACROS |
AC12216–0250 |
| gramine |
ACROS |
AC12018–0100 |
| N-acetylserotonin |
ACROS |
AC22693–1000 |
| melatonin |
ACROS |
AC12536–2500 |
| tryptamine (TRY) |
ACROS |
AC15798–0050 |
| N-methyltryptamine (NMT) |
ACROS |
AC151751000 |
| MDL 100907 (MDL) |
Sigma |
M3324–5MG |
| haloperidol |
Sigma |
H1512 |
| clozapine |
Sigma |
C6305 |
| aripiprazole |
Sigma |
SML0935 |
| fluoxetine hydrochloride |
Sigma |
F132–10MG |
| rizatriptan benzoate |
Sigma |
SML0247–10MG |
| benztropine mesylate |
Sigma |
SML0847–500MG |
| (±)-8-hydroxy-2-(dipropylamino)tetralin hydrobromide (8-OH-DPAT) |
Sigma |
H8520–25MG |
| R-(-)-apomorphine hydrochloride hemihydrate |
Sigma |
A4393–100MG |
| pramipexole hydrochloride |
Sigma |
PHR1598–500MG |
| selegiline hydrochloride |
Sigma |
M003–250MG |
| ladostigil tartrate |
Sigma |
SML2263–5MG |
| RuBi-5-HT |
Tocris |
3856 |
| escitalopram oxalate (ESC) |
Tocris |
4796 |
| L-glutamic acid (GLU) |
Sigma |
G1251–500G |
| g-aminobutyric acid (GABA) |
Sigma |
A5835–25G |
| dopamine hydrochloride (DA) |
Sigma |
H8502–25G |
| norepinephrine bitartrate (NE) |
Sigma |
1468501 |
| U50488 |
Tocris |
0495 |
| dimethyl sulfoxide (DMSO) |
ACROS |
AC327182500 |
| USP grade saline (0.9%) |
VWR |
68099–103 |
| Tetrodotoxin citrate (TTX) |
Tocris |
18660–81-6 |
| Sucrose |
Sigma-Aldrich |
S5016–1KG |
| HBSS |
Fisher |
14175103 |
| MgCl |
Sigma |
M8266–1 KG |
| CaCl2
|
Sigma |
C5670–50G |
| DMEM |
Fisher |
11995073 |
| Puromycin |
A11138–03 |
A11138–03 |
| Poly-D-Lysine |
Sigma |
P6407–5MG |
| Laminin |
Sigma |
L2020 |
| dPBS |
ThermoFisher |
14190–250 |
| Fetal Bovine Serum (FBS) |
Fisher |
26–140–079 |
| Penicillin-streptomycin |
Fisher |
15140–163 |
| Carprofen |
MWI |
24751 |
| Buprenorphine |
MWI |
O56163 |
| Glutamate |
Sigma |
G1251 |
| GABA |
Sigma |
A5835 |
| Dopamine |
Sigma |
H8502–25G |
| Norepinephrine |
Sigma |
74480 |
| Escitalopram |
Tocris |
4796 |
| Serotonin |
Enzo |
ALX-550–328-G001 |
| Kanamycin |
Fisher |
BP906–5 |
| Avertin |
Self-made |
|
| 2,2,2-Tribromoethanol |
Fisher |
AC421430500 |
| tert-Amyl Alcohol (Certified) |
Fisher |
A730–1 |
| 2xYT |
Self-made |
|
| Tryptone |
Fisher |
BP1421–500 |
| Yeast Extract |
Gibco |
DF0127–17-9 |
| Critical Commercial Assays |
| DNA miniprep kit |
Qiagen |
27104 |
| PCR purification kit |
Qiagen |
28104 |
| Endo-free Plasmid Maxi kit |
Qiagen |
12362 |
| Endo-free Plasmid Maxi kit |
Qiagen |
12362 |
| Qiagen Effectene Transfection kit |
Qiagen |
301427 |
| Deposited Data |
| Raw and analyzed data |
This Paper |
https://github.com/lintianlab/psychLight. |
| Experimental Models : Cells Lines |
| HEK293T Cells |
ATCC |
CRL-3216 |
| PSYLI2 |
This paper |
N/A |
| Experimental Models: Organisms/ Strains |
| E18 Sprague Dawley |
Charles River Laboratory |
Strain code: 400 |
| C57BL/6J mice |
Jackson Laboratory |
000664 |
| VMAT2 heterozygous (VMAT2-HET) mice |
William C. Wetsel Lab |
N/A |
| Recombinant DNA |
| pLVX plasmid with EF1a promotor |
Tian Lab |
N/A |
| pCMV_delta8.2 |
Addgene |
12263 |
| pCMV_VSV_G |
Addgene |
8454 |
| pAAV_hSyn |
Addgene |
111068 |
| PCMV |
Addgene |
111054 |
| pCMV_psychLight1 |
This paper |
N/A |
| pCMV psychLight2 |
This paper |
N/A |
| pAAV_hSyn_psychLight2 |
This paper |
N/A |
| Sequence of psychLight2 |
This paper |
N/A |
| pCMV_dLight1.1 |
Tian Lab |
N/A |
| pCMV_dLight1.5 |
Tian Lab |
N/A |
| pCMV_dLight1.4 |
Tian Lab |
N/A |
| pCMV_a2AR sensor |
Tian Lab |
N/A |
| pCMV_nLight3.1 |
Tian Lab |
N/A |
| pCMV_kLight1.2a |
Tian Lab |
N/A |
| pCMV_GRAB5HT1.0 |
Subcloned from Addgene |
140552 |
| Software and Algorithms |
| ImageJ |
ImageJ |
2.0.0-rc-69/1.52p |
| MATLAB R2019b |
Mathworks |
RRID:SCR_001622 |
| AnyMaze |
Stoelting |
6.2 |
| Graphpad Prism 8 |
Graphpad |
8.4.2(464) |
| SparkControl |
Tecan |
2.3 |
| Other |
| 2-photon Microscope |
Scientifica |
|
| Vibrating Microtome |
Leica |
VT1200 |
| Small Animal Stereotax |
David Kopf Instruments |
1900 |
| Zeiss LSM 710 Confocal |
Zeiss |
LSM 710 |
| Fluorescent Platereader |
Tecan |
Spark |
