Table 1.

Summary of MDL protein–protein interaction data^a

Protein 2	Protein 1
	MDL1	MDL2	MDL3
MDL1	Y2H: (yes) co-IP (Nb): yes split luc: yes	Y2H: yes co-IP (Nb): yes split luc: yes	Y2H: no co-IP (Nb): no split luc: no
MDL2	Y2H: yes co-IP (Nb): yes split luc: yes co-IP (At): yes	Y2H: no co-IP (Nb): (yes) split luc: yes co-IP (At): yes	Y2H: no co-IP (Nb): no split luc: yes co-IP (At): no
MDL3	Y2H: no co-IP (Nb): no split luc: no	Y2H: no co-IP (Nb): no split luc: no	Y2H: no co-IP (Nb): yes split luc: yes

^aY2H, yeast two-hybrid assay; co-IP (Nb), co-immunoprecipitation in N. benthamiana; split luc, split luciferase assay; co-IP (At), co-immunoprecipitation in A. thaliana. Protein 1 refers to the MDL bait protein in Y2H analysis, the mCherry-tagged MDL protein in co-IP, and the nLuc-tagged MDL protein in the split luciferase assay. Protein 2 refers to the MDL prey protein in Y2H analysis, the FLAG-tagged MDL protein in co-IP (Nb), the cLuc-tagged MDL protein in the split luciferase assay, and the endogenous MDL2 protein in co-IP (At). Yes, clear interaction; (yes), weak interaction; no, lack of detectable interaction.