Fig. 5.

The ultrawide detection bandwidth of multispectral RSOM divided into low (LF) and high frequency (HF) bands. (A) Representative cross-sectional multispectral RSOM images at various time points (i to vii) of venous and arterial occlusion challenges with concurrent visualization of LF band (11 – 33 MHz) in red and HF band (33–99 MHz) in green at single wavelength 532 nm. Bigger vascular structures are shown in LF band while smaller vascular structures in HF band. Plots of unmixed Hb, HbO₂ and HbT optoacoustic signals for (B) LF and (C) HF bands for the subjects (n = 6) over the occlusion challenges period (mean ± SEM). Scale bar; 500 μm.