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. 2021 Apr 21;22(9):4345. doi: 10.3390/ijms22094345

Table 1.

Enzymatic activity of VvROMT alanine variants 1.

Enzyme % Conversion from Resveratrol % Conversion from Pinostilbene
Pinostilbene Pterostilbene Pterostilbene
Wt 1.6 ± 0.1 30 ± 3.3 99.6 ± 0.1
W20A 1.5 ± 0.2 n.c. 2 20.1 ± 1.5
F24A 2 ± 0.2 n.c. 4.2 ± 1.8
F311A 3.2 ± 0.5 n.c. 25.1 ± 4.1
F318A 1.8 ± 0.1 6.5 ± 1.2 49.9 ± 12.2

1 Reaction conditions: 65 µM of stilbene, 1.25 µM of the purified enzyme, and 400 µM of SAM. The reaction was incubated at 30 °C for 16 h in darkness, values represent averages of 4 replicates ± SD.2 n.c.: not converted.