Table 1.
Enzyme | % Conversion from Resveratrol | % Conversion from Pinostilbene | |
---|---|---|---|
Pinostilbene | Pterostilbene | Pterostilbene | |
Wt | 1.6 ± 0.1 | 30 ± 3.3 | 99.6 ± 0.1 |
W20A | 1.5 ± 0.2 | n.c. 2 | 20.1 ± 1.5 |
F24A | 2 ± 0.2 | n.c. | 4.2 ± 1.8 |
F311A | 3.2 ± 0.5 | n.c. | 25.1 ± 4.1 |
F318A | 1.8 ± 0.1 | 6.5 ± 1.2 | 49.9 ± 12.2 |
1 Reaction conditions: 65 µM of stilbene, 1.25 µM of the purified enzyme, and 400 µM of SAM. The reaction was incubated at 30 °C for 16 h in darkness, values represent averages of 4 replicates ± SD.2 n.c.: not converted.