Figure - PMC

Skip to main content

An official website of the United States government

Here's how you know

Here's how you know

Official websites use .gov
A .gov website belongs to an official government organization in the United States.

Secure .gov websites use HTTPS
A lock ( ) or https:// means you've safely connected to the .gov website. Share sensitive information only on official, secure websites.

View full-text article in PMC

. 2021 Apr 22;13(9):2009. doi: 10.3390/cancers13092009

Search in PMC
Search in PubMed
View in NLM Catalog
Add to search

© 2021 by the authors.

Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).

PMC Copyright notice

Internalization and translation of cancer EV-delivered VEGFA-GFP mRNA by HUVECs. (A) MDA231 cells were stained with PKH26 and transfected with VEGFA-GFP, as shown in (B). This stained MDA231 was then incubated in serum-free culture medium to generate EVs. These EVs (50 µg/mL) were used to treat HUVECs for 24 h. (C) After 24-h co-culture with the PKH26⁺ VEGFA-GFP mRNA⁺ EVs derived from (B), HUVECs internalized the PKH26⁺ EVs and synthesized VEGFA-GFP protein from the EV-mRNA. Scale bars = 20 µm for (B,C). (D) No detectable level of VEGFA-GFP protein was harbored by the PKH26⁺ EVs derived from (B). Scale bars = 5 µm for (D). (E) DNA gel electrophoresis showed that the VEGFA-GFP⁺ MDA231 transferred VEGFA-GFP mRNAs to HUVECs through EVs.

Internalization and translation of cancer EV-delivered VEGFA-GFP mRNA by HUVECs. (A) MDA231 cells were stained with PKH26 and transfected with VEGFA-GFP, as shown in (B). This stained MDA231 was then incubated in serum-free culture medium to generate EVs. These EVs (50 µg/mL) were used to treat HUVECs for 24 h. (C) After 24-h co-culture with the PKH26⁺ VEGFA-GFP mRNA⁺ EVs derived from (B), HUVECs internalized the PKH26⁺ EVs and synthesized VEGFA-GFP protein from the EV-mRNA. Scale bars = 20 µm for (B,C). (D) No detectable level of VEGFA-GFP protein was harbored by the PKH26⁺ EVs derived from (B). Scale bars = 5 µm for (D). (E) DNA gel electrophoresis showed that the VEGFA-GFP⁺ MDA231 transferred VEGFA-GFP mRNAs to HUVECs through EVs.