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. 2021 Apr 22;22(9):4366. doi: 10.3390/ijms22094366

Figure 1.

Figure 1

Peripheral cannabinoid receptor 1 (CB1R) antagonist increased oxygen consumption rate (OCR). 3T3-L1 adipocytes were treated with AM6545, rimonabant (RIM), and isoproterenol (ISO) at 4 and 48 h. OCR was measured in basal conditions or in response to sequential treatment with 2 oligomycin, 0.75 FFCP (respiratory chain uncoupler), and 1 µM rotenone/antimycin A (inhibitor of respiratory chain complex I and complex III) using the Seahorse XF-24 analyzer. (A) Mitochondrial respiration curves at 4 h after treatment. (B) Parameters calculated from the tracing at 4 h after treatment. (C) Mitochondrial respiration curves 48 h after treatment. (D) Parameters calculated from the OCR at 48 h after treatment. Data on graphs are presented as the mean ± standard error of mean (SEM) of 4 independent rounds of the cells; * p < 0.05 vs. control, ** p < 0.01 vs. control.