Submandibular glands were collected from C57BL/6 wild type (WT), AppNL–G–F, APP/PS1, and APP−/− male and female mice, fixed in 4% paraformaldehyde, and serially sectioned (10μm). Sections were immunostained using anti-Aβ antibody and antibody binding was visualized using Vector VIP as the chromogen. Representative images at 20X are shown from n=2–4 C57BL/6 wild type (WT), AppNL–G–F, APP/PS1 mice with a male and female APP−/− gland used as an antibody specificity control.