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. 2021 Apr 22;26(9):2434. doi: 10.3390/molecules26092434

Figure 2.

Figure 2

Effect of CT treatment on the metabolic gene expression levels in adipose tissues. One week after acclimatization, C57BL/6J mice were fed HFD mixed with 0.25% and 0.5% CT for 7 weeks. The mRNA expression levels of (a) sterol regulatory element-binding protein 1c (SREBP-1c), peroxisome proliferator-activated receptor gamma (PPARγ), CCAAT/enhancer-binding protein alpha (C/EBPα), acetyl-CoA carboxylase-1 (ACC1) and fatty acid synthase (FAS) (b) tumor necrosis factor alpha (TNFα) (c) hormone sensitive lipase (HSL), carnitine palmitoyltransferase 1a (CPT1a) and peroxisome proliferator-activated receptor alpha (PPARα) were measured in epididymal fat pads by quantitative real-time PCR (qRT-PCR). (d) The mRNA levels of CPT1a and uncoupling protein 1 (UCP-1) were analyzed with in brown adipose tissues by qRT-PCR. NC: normal chow; (-): HFD; CT-HFD; CT treated HFD. Values are mean ± SE. AVOVA p-value: ** <0.01, * <0.05 vs. NC group; ## <0.01,# <0.05 vs. HFD-fed group.