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. 2021 Apr 22;26(9):2434. doi: 10.3390/molecules26092434

Figure 4.

Figure 4

Effect of CT treatment on hepatic steatosis. One week after acclimatization, C57BL/6J mice were fed HFD mixed with 0.25% and 0.5% CT for 7 weeks. After 7 weeks, (a–c) Liver mass, hepatic TG level and hepatic cholesterol levels were measured. (d) H&E staining was performed on liver sections. The mRNA levels of (e) sterol regulatory element-binding protein 1c (SREBP-1c) and peroxisome proliferator-activated receptor gamma (PPARγ) (f) carnitine palmitoyltransferase 1a (CPT1a) and peroxisome proliferator-activated receptor alpha (PPARα) (g) tumor necrosis factor alpha (TNFα) and macrophage marker F4/80 were analyzed in liver by RT-qPCR. NC: normal chow; (-): HFD; CT-HFD; CT treated HFD. Values are mean ± SE. AVOVA p-value: ** <0.01, * <0.05 vs. NC group; ## <0.01, # <0.05 vs. HFD-fed group.