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. 2021 Apr 22;26(9):2434. doi: 10.3390/molecules26092434

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© 2021 by the authors.

Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).

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Antioxidant effect of CT on HFD-fed mice. One week after acclimatization, C57BL/6J mice were fed HFD mixed with 0.25% and 0.5% CT for 7 weeks. The liver mRNA expression level of (a) heme oxygenase-1 (HO-1) and nuclear factor, erythroid 2 like 2 (Nrf2) were analyzed by RT-qPCR. (b) The protein expression levels of HO-1 and Nrf2 were estimated by western blotting and normalized to β-actin. (c) The level of thiobarbituric acid reactive substances (TBARS) were analyzed in liver lysate. NC: normal chow; (-): HFD; CT-HFD; CT treated HFD. Values are mean ± SE. AVOVA p-value: * <0.05 vs. NC group; ^# <0.05 vs. HFD-fed group.