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. 2021 Apr 22;13(9):2022. doi: 10.3390/cancers13092022

Figure 1.

Figure 1

Levels of stemness and EMT markers in H1975 NSCLC cells grown in adhesion or as tumor spheres, and response to EGFR inhibitors. (A) Levels of CD44, vimentin, OCT3/4, and E-cadherin in H1975 parental cells and H1975 tumor spheres (H1975-TS). (B) Cell toxicity assay in parental cells and H1975-TS exposed to increasing concentrations of erlotinib and WZ4002 for 72 h. (C) Levels of EGFR downstream signaling mediators and effectors in response to treatment with EGFR inhibitors (0.5 or 1 µmol/L) for 48 h. (D) Levels of total EGFR, p-EGFRTyr1068, and EGFR_L858R in parental cells and tumor spheres at different serial passages. At least three independent experiments were performed. Actin, GAPDH, or tubulin were used to ensure equal loading.