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. 2021 May 15;40:171. doi: 10.1186/s13046-021-01978-8

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© The Author(s) 2021

Open AccessThis article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated in a credit line to the data.

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Fig. 2 — Knockdown of SRSF1 suppresses oncogenic roles in vitro and in vivo. a Luminal subtype cell line MCF7 or triple negative breast cancer cell line MDA-MB-231 are transfected with SRSF1 shRNA plasmid (shSRSF1) or control plasmid (pLKO.1). SRSF1 knockdown efficiency is confirmed by western blot and RT-qPCR. b, c Cell proliferation assay and clonogenic survival assay are performed using cells described in (a). d The cell cycle of cells described in (a) is analyzed by flow cytometry and the relative cell population of each cell cycle phase is quantified in the bar graph. e The ration of apoptosis in each group is calculated by flow cytometry. f Measurement of cell migration by wound-healing assays and transwell assays using cells described in (a). g T47D/sh-SRSF1 cells or control T47D/pLKO.1 cells are transplanted to nude mice. Tumors derived from T47D/sh-SRSF1 or control cells are excised and presented. h Volume of tumors are calculated and plotted. i Representative IHC results of SRSF1 and Ki67 in xenografts are presented (200*). *, P < 0.05; ** P < 0.01; *** P < 0.001; **** P < 0.0001