Fig. 4.

Motif analysis of SRSF1-affected AS events. a Western blot for testing the affinity of the SRSF1antibody to the SRSF1. b RIP-PCR results of representative SRSF1-regulated SE events. c, d Flowchart of the potential SRSF1 motifs derived from the 6-mers overrepresented in the activated (c) exon and the flanking constitutive exon of the repressed (d) cassette exon based on the RNA-seq data. e, f In vivo CLIP assay is performed and analyzed by RT-PCR with primer pairs complementary to SRSF1-affected cassette exons and two flanking constitutive exons