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. 2021 Apr 21;26(9):2422. doi: 10.3390/molecules26092422

Figure 3.

Figure 3

Figure 3

Garcinielliptone G (5) induces apoptosis. (A) Cells were treated with 10 µM garcinielliptone G (5) for 24 h, stained with APC Annexin/7-ADD, and analyzed by flow cytometry. Etoposide (1 µM) was used as a positive control. (B) and (C) THP-1 cells were treated with 10 µM garcinielliptone G (5) for 24 h, and cell lysates were prepared. Cleaved caspase 3, cleaved PARP, procaspase 8, and procaspase 9 protein levels were determined by western blot analysis. β-actin expression was determined to confirm equal protein loading. Values are presented as the mean ± SD of three independent experiments. * p < 0.05, ** p < 0.01.