Figure 1.

Graphical representation of experimental methodology with corresponding timelines. Human liver microtissues (MTs) are generated using HepaRG, hTERT-HSC, and THP-1. Initial experiments focused on optimization of dissociation using Liberase, Accutase and Accumax by comparing yield and viability. After dissociation, cells were suspended in 96 well plates to assess the maintenance of crucial cellular characteristics: HepaRG were able to release albumin; hTERT-HSC were able to become activated upon exposure to TGF-β1 (A). The optimized dissociation protocol was used to dissociate TGF-β1 treated and untreated MTs for processing following the single cell sequencing workflow (B).