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. 2021 Apr 22;22(9):4372. doi: 10.3390/ijms22094372

Figure 2.

Figure 2

Cell dissociation outcome. MTs were generated using differentiated HepaRG, differentiated THP-1 and hTERT-HSCs. MTs were allowed to aggregate over 72 h and then maintained in FBS-free medium for 3 or 9 days. Dissociation was carried out using Accutase, Liberase or Accumax at day 3 (A) and 9 (B) of maintenance. Trypan blue was used to assess the percentage of viability of the cells immediately after dissociation and cell number was recorded in order to calculate the cell yield percentage from the MTs by comparing cell number following dissociation to cell number used to generate the MTs. Stress markers (HMOX1 and NQO1) and hepatic markers (ALB, CD44 and CD68) were investigated using q-RT-PCR directly after dissociation at day 3 (C) and day 9 (D) and compared to undissociated MTs (baseline). Data represent mean ± SD (n = 6). Statistical analysis using unpaired student’s t-test; *: p ≤ 0.05; **: p ≤ 0.01; ***: p ≤ 0.001.