Table 3.

Method for dissociation.

Reagent	Enzyme Concentration or Quantity	Dissociation Time Day 3	Dissociation Time Day 9	Shaken by Hand
Liberase	1.3 U/mL	40 min	60 min	10× every 20 min
Accumax	900 µL	50 min	60 min	10× every 20 min
Accutase	900 µL	60 min	60 min	10× every 20 min