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. 2021 Apr 22;22(9):4373. doi: 10.3390/ijms22094373

Figure 2.

Figure 2

Effect of resveratrol (RSV), tunicamycin (TM) and TM and RSV on markers of ER stress in FRTL-5 cells. After reaching confluence, cells were treated with either 0.1% DMSO (control), 10 µM RSV, 0.1 µg/mL TM or 10 µM RSV and 0.1 µg/mL TM in 6H medium for 6 and 48 h. (A,B) Relative mRNA levels (A) and protein levels (B) of HSPA5 and DDIT3 are expressed as fold of control. (B) Representative immunoblots for HSPA5 and DDIT3 including immunoblots for β-Actin and Vinculin as internal controls are shown on the right. (A) Data from qPCR are means and SD calculated from three replicates for the same treatment of three independent experiments and were subjected to 2-factorial (T = treatment, E = experiment) ANOVA. (B) Data from immunoblotting are means and SD calculated from one replicate for the same treatment of three independent experiments and were subjected to 1-factorial ANOVA. Bars without the same letters (a,b,c, or A,B,C) differ (p < 0.05). (C) Representative image from agarose gel electrophoresis of unspliced (192 bp PCR product) and spliced (s) XBP1 (166 bp PCR product) as detected by conventional PCR. The mRNA expression of ACTB served as an internal control. Abbreviation: n.d., not detectable.