Figure 4.

Effect of resveratrol (RSV), tunicamycin (TM) and TM and RSV on protein levels of genes involved in thyroid hormone synthesis and iodide uptake in FRTL-5 cells. After reaching confluence, cells were treated with either 0.1% DMSO (control), 10 µM RSV, 0.1 µg/mL TM or 10 µM RSV and 0.1 µg/mL TM in 6H medium for 48 h. (A,C,F) Relative protein levels of glycosylated and deglycosylated NIS (A), deglycosylated NIS (C) and glycosylated and deglycosylated TG (F) are expressed as fold of control. Representative immunoblots including immunoblots for β-Actin and Vinculin as internal controls are shown. Data from immunoblotting are means and SD calculated from one replicate for the same treatment of three independent experiments and were subjected to 1-factorial ANOVA. (B,D,G) Immunoblots for glycosylated and deglycosylated NIS (B), deglycosylated NIS (D) and glycosylated and deglycosylated TG (G) following treatment of cell protein without (−) or with (+) Peptide-N-Glycosidase F (PNGase F). (E) Iodide uptake determined in the absence (−) and presence (+) of the NIS inhibitor KClO₄. Data are expressed as pmol iodide/mg protein and are means and SD from one independent experiment performed in triplicate. (A,C,E,F) Bars without the same letters (^{a,b,c, or A,B,C}) differ (p < 0.05). Abbreviation: n.d., not detectable.