Table 1.
Characteristics of the gene-specific primers used for qPCR analysis.
| Gene Name | Primer Sequence (Forward, Reverse) | Product Size (bp) | NCBI GenBank |
|---|---|---|---|
| Reference Genes | |||
| ACTB | GACCTCTATGCCAACACAGT CACCAATCCACACAGAGTAC | 154 | NM_031144 |
| RPL13 | CTTAAATTGGCCACGCAGCT CTTCTCAACGTCTTGCTCTG | 198 | XR_086310 |
| TOP1 | GAAGAACGCTATCCAGAAGG GCTTTGGGACTCAGCTTCAT | 137 | NM_022615 |
| YWHAZ | GACGGAAGGTGCTGAGAAA GCAGCAACCTCAGCCAAGT | 198 | NM_013011 |
| Target Genes | |||
| DDIT3 | ACAAGCACCTCCCAAAGCCC TGCTCCTTCTCCTTCATGCGC |
155 | NM_001109986 |
| HMOX1 | AGCATGTCCCAGGATTTGTC TCACCAGCTTAAAGCCTTCC |
130 | NM_012580 |
| HSAP5 | TCAGCCCACCGTAACAATCAAGG TCCTCAGCAAACTTCTCGGCG |
282 | NM_013083 |
| NIS | GCTGTGGCATTGTCATGTTC TGAGGTCTTCCACAGTCACA |
219 | NM_052983 |
| PAX-8 | CCTTACTCAACAGTACCCTGG AGCTAGAACTGGAGAGCTCTG |
162 | NM_031141 |
| SOD1 | TATGGTGGTCCACGAGAAAC AATCACACCACAAGCCAAGC |
100 | NM_017050 |
| TG | GTTCCTACGTGTACTAGTGAG CATACTGGAGTTGGAGAGCAG |
196 | NM_030988 |
| TPO | CAGGTGTTGAGAAGCAGTTG CTTTGAAAGCTGTAGCCAGG |
255 | NM_019353 |
| TSHR | CCAGAAGCTTGACTTACATAG CATGTAAGGGTTGTCTGTGAT |
161 | NM_012888 |
| TTF-1 | GCATGAATATGAGCGGCATGG ACTTCTGCTGCTTGAAGCGTC |
153 | NM_013093 |
| TTF-2 | GAAGTGGCAGAACAGCATCC AGCTGCCGCTCTCGAACATG |
139 | NM_138909 |
| XBP1 | GACACGCTTGGGGATGAATGC AGAGGCAACAGCGTCAGAATCC |
192/166 |
NM_001004210 (XBP1) NM_001271731 (sXBP1) |