Table 1.

IMC melanoma signaturing. Immunophenotyping of protein expression in WM115 (primary) and WM266-4 (metastatic) human melanoma cells.

		WM115			WM266-4
	IF (phenotype)	Global Expression (100%)	Absence of Expression	Expression in Sub-Populations (%)	Global Expression (100%)	Absence of Expression	Expression in Sub-Populations (%)
Proteins
Vimentin		+			+
N-Cadherin				66		+
E-Cadherin			+			+
ZEB1				77			85
ZEB2				14			80
SLUG				63 (54: nuclear strong signal)			69 (3: nuclear strong signal)
p63α			+			+
PRRX1				77			70
Keratin-5		+			+
Keratin-8/18		+			+
PDGFRβ				74			76
LOXL2				40			46
p-SMAD2		+			+
SOX2		+					10
SOX9		+			+
SOX10				2			11
p-STAT3				87			87
PCM1				83	+
TRA1-60(S)		+		1.7 (strong signal)	+
p-AKT		+			+
p-GSK3β		+			+
p-ERK1/2		+			+
p-p38				90	+
p-S6		+			+
β-Catenin		+			+
YAP				98			68
ATF4		+			+
CHOP		+			+
MCT1				97	+
MCT4				95	+
HIF1α			+				66
p-p53Ser15				7			7
p-p53Ser37				31			42
p-H2AX				48			44
a-Caspase-3			+			+
DR5		+			+
ATG12		+			+