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. 2021 Apr 22;13(9):2032. doi: 10.3390/cancers13092032

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© 2021 by the authors.

Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).

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(A) Administration procedure of the xenograft and treatment. (B) Xenografts of T24-GCB cells were treated with the PBS control, EA (4.5 mg/kg), GCB (1 mg/kg) and EA (4.5 mg/kg) + GCB (1 mg/kg), and in vivo tumors and dissected tumor masses were aligned. (C) Curves of tumor size, (D) tumor weight, (E) mouse body weight and (F) liver (AST and ALT) and kidney (BUN, CRE and UA) function among the treated groups. (G) Immunohistochemical staining of Smad2, Smad3 and VEGF-A and H&E staining (the black arrows indicate intratumoral vessels) of xenograft tumors among the treated groups. All statistical tests were analyzed with Student’s t test with significance at * < 0.05 and *** < 0.001.