Figure 4.
Effect of antipsychotics on the mTORC1 signaling pathway. Expression of the mTORC1 signaling pathway was assessed using Western blot. There were significant differences in p70S6K activity between control cells and those treated with antipsychotics (F3, 12 = 1.840, p < 0.0001). Control BV-2 microglial cells exhibited moderate activity of p70S6K (a,b), a downstream protein in the mTORC1 signaling pathway. Haloperidol (p < 0.0001) and risperidone (p < 0.0001) increased the expression of phosphorylated p70S6K (p-p70S6K), indicating mTORC1 activity. On the other hand, aripiprazole (p < 0.0001) significantly reduced the p-p70S6K/p70S6K ratio, indicating diminished mTORC1 activity compared to the control cells (b). * Statistical significance was set at p ≤ 0.05 values.