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. 2021 Apr 22;22(9):4387. doi: 10.3390/ijms22094387

Figure 6.

Figure 6

Western blot analysis of AKT pathway activation. Protein expression pan AKT, pan pAKT, i AKT and i pAKT in co-culture cells treated with trametinib (25 nM) and celecoxib (50 nM). WB bands were analyzed by densitometry and results were normalized to GAPDH. The left panel illustrates WB bands (Ctrl = control, T = trametinib, C = celecoxib, T + C = trametinib + celecoxib), while right panels illustrate the quantitative analysis of WB results (1 = control, 2 = trametinib, 3 = celecoxib, 4 = celecoxib + trametinib, 5 = AKT pathway inhibitor + celecoxib + trametinib). **** p < 0.0001. Each bar represents mean ± standard deviation (n = 3).