KEY RESOURCES TABLE
| REAGENT or RESOURCE | SOURCE | IDENTIFIER |
|---|---|---|
| Antibodies | ||
| Alexa Fluor 647 anti-CD49f | Bio-Legend | 313610; RRID:AB_493637 |
| Armenian Hamster anti-Ccl2 | Bio X Cell | BE0185; RRID:AB_10950302 |
| Chicken anti-K14 | Bio-Legend | Poly9060; RRID:AB_2565055 |
| Chicken anti-K15 | Bio-Legend | 833904; RRID:AB_2616894 |
| Donkey Anti-Chicken Cy5 | Jackson | 703-175-155; RRID:AB_2340365 |
| Donkey anti-Rabbit AF 488 | Jackson | 711-546-152; RRID:AB_2340619 |
| Donkey anti-Rabbit AF594 | Jackson | 711-585-152; RRID:AB_2340621 |
| Donkey anti-Rat Cy2 | Jackson | 703-175-155; RRID:AB_2340365 |
| FITC anti-CD34 | BD Biosciences | 560238; RRID:AB_1645242 |
| Goat anti-8-OHdG | Novus | NB600-1508SS; RRID:AB_787860 |
| Goat anti-Chicken AF594 | Jackson | 103-585-155; RRID:AB_2337391 |
| Goat anti-Rabbit HRP | Abcam | Ab6721; RRID:AB_955447 |
| Goat anti-Rat AF594 | Jackson | 112-585-155 |
| Mouse anti-Erk2 | Santa Cruz Biotechnology | SC-1647; RRID:AB_627547 |
| Rabbit anti-Arginase 1 | Protein Tech | 16001-1-AP; RRID:AB_2289842 |
| Rabbit anti-beta Actin | Abcam | Ab8227; RRID:AB_2305186 |
| Rabbit anti-Cleaved Casp3 | Abcam | Ab2302; RRID:AB_302962 |
| Rabbit anti-Keratin 10 | Bio-Legend | Poly 19054; RRID:AB_2616955 |
| Rabbit anti-Nqo1 | Abcam | Ab34173; RRID:AB_2251526 |
| Rabbit anti-Nrf2 phospho. S40 | Abcam | Ab76026; RRID:AB_1524049 |
| Rabbit anti-SOD2 | Abcam | Ab13533; RRID:AB_300434 |
| Rabbit IgG | Abcam | Ab172569 |
| Rat anti-Ccl2 | R&D Biosystems | Mab479-100; RRID:AB_2259770 |
| Rat anti-CD31 | BD Biosciences | 553370; RRID:AB_394816 |
| Rat anti-F4/80 | Abcam | Ab6640; RRID:AB_1140040 |
| Rat anti-Ki-67 | Bio-Legend | 652401; RRID:AB_11203533 |
| Rat anti-Ly-6G | BD Biosciences | 551459; RRID:AB_394206 |
| Chemicals, Peptides and Recombinant Proteins | ||
| CDDO-IM | Torcis | 473710 |
| Hoechst 33342 | Thermo Fisher | 62249 |
| M-CSF | Millipore | M9170 |
| Mifepristone | Sigma-Aldrich | M8046-500mg |
| PD 153035 | Abcam | Ab141839 |
| Recombinant Mouse Ccl2 | R&D Biosystems | 479-JE |
| Tamoxifen | Sigma-Aldrich | T5648-5G |
| Critical Commercial Assays | ||
| High Capacity cDNA Kit | Thermo Fisher | 4368814 |
| Lineage Cell Depletion Kit, mouse | Miltenyi | 130-090-858 |
| Lipofectamine 3000 | Invitrogen | L3000015 |
| MACS Dead Cell Removal Kit | Miltenyi | 130-090-101 |
| Nugen Trio RNA-seq Kit | Nugen | 0507 |
| QIA-Quick PCR Purification Kit | QIAGEN | 28104 |
| RN-Easy Plus Micro Kit | QIAGEN | 74034 |
| siRNA Silencer (mouse Ccl2) | Thermo Fisher | AM16708; ID151586 |
| siRNA Silencer (negative control) | Thermo Fisher | AM4611 |
| SSO Adv. Univ. SYBR Green Super Mix | Bio-Rad | 172-5271 |
| Deposited Data | ||
| Bulk RNA-seq data | This paper | GEO Series accession: GSE159361 |
| Experimental Models: Organisms/Strains | ||
| C57BL/6J | Jackson Laboratory | 000664 |
| BKS.Cg-Dock7m =/ = Lepr db/J | Jackson Laboratory | 000642 |
| Tg(KRT14-cre/ERT)20Efu/J | Jackson Laboratory | 005107 |
| B6;SJL-Tg(Krt15-cre/PGR)22Cot | Jackson Laboratory | 005249 |
| Gt(ROSA)26Sortm4(ACTB-tdTomato,-EGFP)Luo/J | Jackson Laboratory | 007676 |
| Nrf2flox/flox | Gift from Dr. Shyam Biswal | N/A |
| Oligonucleotides | ||
| Nqo1 ChIP ARE site F: 5′-CACTCAGCCGTGGGAAGT-3′ | Life Technologies | N/A |
| Nqo1 ChIP ARE site R: 5′-AGCAGAACGCAGCACGAAT-3′ | Life Technologies | N/A |
| Nqo1 ChIP NS site F: 5′-TCTGGGGACTTGGGTATCTG-3′ | Life Technologies | N/A |
| Nqo1 ChIP NS site R: 5′-TACGCTGTAGTGGTGGTGGA-3′ | Life Technologies | N/A |
| Ccl2 ChIP ARE site F: 5′-ACTACTGCCTCCAA TCCTGAAATAA-3′ | Life Technologies | N/A |
| Ccl2 ChIP ARE site R: 5′-GGACCCTCCTTTTGTTACCT-3′ | Life Technologies | N/A |
| 18 s F: 5′-CGGCTACCAGATCCAAGGAA-3′ | Life Technologies | N/A |
| 18 s R: 5′-GCTGGAATTACCGCGGCTG-3′ | Life Technologies | N/A |
| Ccl2 F: 5′-GCAGCAGGTGTCCCAAAGAA-3′ | Life Technologies | N/A |
| Ccl2 R: 5′-TTGGTTCCGATCCAGGTTTTT-3′ | Life Technologies | N/A |
| Please refer to Table S1 for complete listing of oligonucleotides used in the study. | N/A | |
| Software and Algorithms | ||
| Cluster 3.0 | (de Hoon et al., 2004) | http://bonsai.hgc.jp/~mdehoon/software/cluster/ |
| Enrichr | (Kuleshov et al., 2016) | https://maayanlab.cloud/Enrichr/ |
| Gene Set Enrichment Analysis | (Subramanian et al., 2005) | https://www.gsea-msigdb.org/gsea/index.jsp |
| Graph pad Prism 8 | Graph pad | N/A |
| ImageJ /FIJI | (Schneider et al., 2012) | https://imagej.nih.gov/ij/ |
| Java Tree-View | (Saldanha, 2004) | http://jtreeview.sourceforge.net/ |
| RSEM | (Li and Dewey, 2011) | https://deweylab.github.io/RSEM/ |
| STAR Aligner | (Dobin et al., 2013) | https://code.google.com/archive/p/rna-star/ |
| Trimmomatic | (Bolger et al., 2014) | http://www.usadellab.org/cms/?page=trimmomatic |
| Other | ||
| Accutase | Bio-Legend | 42301 |
| Dispase | Thermo Fisher | 17105041 |
| DMEM: F12 (1:1) | Thermo Fisher | 11330032 |
| Citri-Solve | Fisher | 04355121 |
| DAKO Target Retrieval Solution | DAKO | S1700 |
| Dynabeads Protein A | Thermo Fisher | 10001D |
| Ethanol | Sigma | E7023 |
| Glass-bottom Micro-well dishes | Mat-Tek | P35G-1.5-14-C |
| IMMPACT DAB | Vector Laboratories | SK-4105 |
| KAPA Hot-Start Ready Mix with Dye | KAPA Biosystems | KK5609 |
| KBM-Gold Basal Growth Medium | Lonza | 192060 |
| Matrigel Membrane Matrix | BD Biosciences | 354230 |
| Protein Assay Reagent | Bio-Rad | 5000006 |
| Proteinase K | QIAGEN | 1017738 |
| PVDF Immobilon-FL membranes | Millipore | IPF00010 |
| Silicone Isolator Sheets | Grace Bio-Labs | 665581 |
| Trizol | Life Technologies | 15596018 |
| Normal Donkey Serum | Jackson | 017-000-121 |
| Normal Goat Serum | Jackson | 0005-000-121 |
| SSO-Adv. Universal SYBR Green Super mix | Bio-Rad | 172-5271 |