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. 2021 Apr 23;22(9):4413. doi: 10.3390/ijms22094413

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© 2021 by the authors.

Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).

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(A) Schematic representation of the GJA1 transcript encoding Cx43 and up to 6 putative N-terminally truncated forms. Suggested internal translation initiation sites corresponding to starting AUG codons, encoding for methionines, are shown as yellow circles. Created with Biorender.com (accessed on 15 April 2021). Modified from [8,577]. (B) Representative Western blot showing expression of full-length Cx43 (P2, P1 and P0 phosphorylation states), together with the putative N-terminally truncated isoforms GJA1-32k, GJA1-29k, GJA1-26k and GJA1-20k, in tissue extracts obtained from a normoxic isolated rat heart.