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. 2021 Apr 23;22(9):4436. doi: 10.3390/ijms22094436

Figure 3.

Figure 3

Quantification of cell mortality and survival after the irradiation of cells pretreated with PtNPs or not. (A) Percentage of dead cells measured by using the PI exclusion method. Cells were irradiated at 6 Gy after being exposed to 0.5 mM NPs or not, and the mortality was evaluated after 3 or 6 days of culture. The experiment was conducted on two independent sets of triplicates for each time point for the MDA-MB-231 cells and on one triplicate for day 3 and two independent sets of triplicates for day 6 for the T47D cells. (B) Measurement of the proliferation by counting the absolute living cell numbers. Cells were irradiated at 6 Gy after being exposed to 0.5 mM NPs or not. Cell counts were assessed by flow cytometry, after 3 or 6 days of culture post-exposure. The experiment was conducted on two independent sets of triplicates for each time point for the MDA-MB-231 cells and on one triplicate for day 3 and two independent sets of triplicates for day 6 for the T47D cells. The statistical significance was determined by using the Kruskal–Wallis test. Note: ns, non-significant; * p < 0.05 and *** p < 0.001.