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. 2021 Apr 23;13(9):2051. doi: 10.3390/cancers13092051

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© 2021 by the authors.

Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).

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Transwell migration assay on 82.3 and HUH28 cells. Cells were seeded on the surface of the migration transwell chamber and separated by a porous membrane. After 48 h of incubation, the membranes were fixed with methanol and stained with crystal violet. The area of the cells that invaded the membrane was calculated using the ImageJ 2 software; five different fields were evaluated. Migration is expressed as the ratio of the mean ± SEM of the area of migrated cells to the area of the same number of cells plated (control). **** p < 0.00001.