Skip to main content
. 2021 Apr 23;22(9):4439. doi: 10.3390/ijms22094439

Figure 2.

Figure 2

Metformin protects against NMDA-induced loss of NeuN-positive cells. The number of NeuN-positive cells in the ganglion cell layer (GCL) was counted one day after intravitreal injection of NMDA. (A): Saline + vehicle (a); saline + metformin (b); NMDA + vehicle (c); NMDA + metformin (d). The doses of NMDA and metformin were 200 nmol/eye and 20 nmol/eye, respectively. Scale bar, 50 µm. (B): Bar graph showing the number of NeuN-positive cells per millimeter in the GCL of each retina. n = 5–6. ** p < 0.01; ns: no significance; Kruskal–Wallis test.