Figure 7.

Bestatin treatment does not affect the overall leukocyte populations in the dLNs during lymphedema progression. (A) Number of T cells, B cells, CD11b+ cells, dendritic cells, monocytes, and macrophages in dLNs after single vessel ligation lymphedema surgery for the saline control group and the bestatin-treated group at 1W, 2W, and 3W timepoints. Represented as the sum within the two dLNs. (B) T cell, B cell, CD11b+ cell, dendritic cell, monocyte, and macrophage frequencies of total leukocytes within dLNs after single vessel ligation lymphedema surgery for the saline control group and the bestatin-treated group at 1W, 2W, and 3W timepoints. Represented as the sum within the two dLNs for each leukocyte type divided by the sum of overall leukocytes within the two dLNs. Multiple Mann–Whitney tests using the Holm–Šídák method were used to compare between treatments at each timepoint for both number of cells and percentage of leukocytes. 1W (n = 15), 2W (n = 18), 3W (n = 12). Mean ± s.e.m.