Figure 4.

Effects of crassolide on the ability of lipopolysaccharides (LPS)-stimulated BMDCs from OT-II mice, which produce CD4+ T cells which recognizes OVA_323–339, to induce antigen-specific CD4+ T cell proliferation and cytokines production. (a) Cell proliferation of OT-II CD4+ T cells measured by ³H-TdR incorporation assay after co-culturing with OVA_323–339 (OVAP₂) peptide and LPS-stimulated BMDCs in a 5:1 ratio with or without crassolide treatment for 96 h. (b–d) The amount of cytokines in the culture supernatants measured by ELISA. Data are presented as mean ± SEM of triplicate samples from one of three independent experiments. *** p < 0.001, versus LPS + 0.1% DMSO-treated group, tested by one-way ANOVA followed by Tukey’s multiple comparison test.