Figure 8.

Effects of carssolide on the expression of surface markers of splenic DCs in APS mice. Spleen cells were purified from different mouse groups on day 56, and the relative mean fluorescence intensity (MFI) of (a) CD80 and (b) CD86 were measured by flow cytometry. Data are presented as mean ± SEM of 5 mice from one of three independent experiments. (c) IL-12 and (d) IL-23 mRNA expression levels in purified splenic CD11c+DCs determined by reverse transcription-quantitative PCR. Data were normalized to hypoxanthine guanine phosphoribosyl transferase 1 expression levels. * p < 0.05, ** p < 0.01, versus vehicle-treated APS mice group, tested by one way ANOVA followed by Tukey’s multiple comparison test.