Figure 9.
Effects of crassolide on cell proliferation and cytokines production in the spleen CD4+ T cells of APS mice after Β2GPI stimulation. (a) Spleen was harvested on day 56 and cultured in the presence of Β2GPI for 96 h. (a) Proliferation determined by radioactivity incorporation in count per minute (cpm) after pulsed treatment with 1 μCi of [3H]thymidine per well for the last 18 h. (b,c) The amounts of (b) IFN-γ and (c) IL-17A in the culture supernatants measured by ELISA. (d,e) The percentages of (d) IFN-γ+, (e) IL-17A+ and (f) Foxp3+ cells among CD4+ T cells measured by flow cytometry. Data are presented as mean ± SEM of 5 mice from one of three independent experiments. * p < 0.05, *** p < 0.001, versus vehicle-treated APS mice group, tested by one-way ANOVA followed by Tukey’s multiple comparison test.