Figure 5.

Effect of the identified drugs on the heteroallelic strains. (A) Oxygen consumption rate of the AAC2/aac2^M114P strain grown in SC medium (without uracil) at 37 °C supplemented with 0.6% glucose. (B) Determination of petite frequency of the heteroallelic AAC2/aac2^M114P strain grown in YP medium supplemented with 2% glucose. (C) Oxygen consumption rate of AAC2/aac2^R96H strain grown at 37 °C in YP medium supplemented with 0.6% glucose. (D) Determination of petite frequency of the AAC2/aac2^R96H strain grown in YP medium at 37 °C supplemented with 2% glucose. For the oxygen consumption rate analysis (A,C), values were normalized to the homoallelic (AAC2/AAC2) wild type strain and represented as the mean of at least four independent experiments ± SD. For petite analysis (B,D) more than 4000 colonies/strain were scored. All values are means of three independent experiments. Statistical analysis was performed using an unpaired, two-tailed Student’s t-test comparing treated (blue bars) versus untreated mutant (red bar): * p < 0.05; ** p < 0.01; *** p < 0.001. OTI = otilonium bromide, PER = pergolide mesylate, TRI = trifluoperazine 2HCl, SER = sertraline HCl, BEN = benzydamine HCl.