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. 2021 Apr 26;22(9):4506. doi: 10.3390/ijms22094506

Figure 4.

Figure 4

Effects of XN (20 uM) on ROS formation in C6 cells. (A,B) The C6 cells were pretreated with 500 uM NAC to scavenge the intracellular total ROS, or 10 uM Mito-Tempo to scavenge the mitochondrial ROS, or 10 uM APO to inhibit the NADPH oxidase for 1.5 h before the co-treatment with XN for another 96 h, and the cell viability and death were measured by trypan blue assay. (C) The change of mitochondrial ROS in C6 cells during the initial 20 min of XN incubation was detected by flow cytometry. (D) The change of mitochondrial ROS in C6 cells during the 24, 48, and 72 h of XN incubation was detected by flow cytometry. Mitochondrial ROS were labeled by mitoSOXTM. ** p < 0.01 vs. control groups. All data presented are the mean ± SEM from three independent experiments. ## p < 0.01 vs. XN-treated group.