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Effect of XN on energy metabolism of C6 cells. (A) MTT assay was used to assay the effects of XN treatment on C6 cells within different times. (B,C) XN (20uM) reduced the ATP content and energy charge. Cells were treated with XN (20uM) for 24 h, then the content of ATP, ADP, and AMP were determined by HPLC. All data presented are the mean ± SEM from three independent experiments. * p < 0.05, ** p < 0.01 vs. control groups.
