Figure 10.

Effect of bortezomib on XN (20 uM)-induced inhibition of C6 cell proliferation. The C6 cells were pretreated with 2 nM or 4 nM bortezomib, and inhibitor of the LONP1 for 1.5 h before the co-treatment with XN for another 48 h. (A,B) The cell viability and the cell deaths were measured by SRB assay. (C) The morphology changes were captured by a Leica Microsystems microscope (200× g magnification), and intracellular vacuoles were marked by red arrows. All data presented are the mean ± SEM from three independent experiments. ** p < 0.01 vs. control groups. # p < 0.05, ## p < 0.01 vs. XN-treated group.