Table 2.
Real-time RT-qPCR primer sets for mouse netrins, Sema3A, neogenin, BOC, CDO, Unc5B, Unc5C, MyHCs, and HPRT.
| Primer Name | Primer Sequence (5′ To 3′) Forward | Primer Sequence (5′ to 3′) Reverse | Probe No. |
|---|---|---|---|
| netrin-1 | CCCCTTGCATCAAGATTCC | GGCCTTGCAATAGGAGTCAC | 71 |
| netrin-3 | CCCAGGTTTCCAGCAGAG | GGTCCAGGGACAGGAGTCTTA | 2 |
| netrin-4 | CAGCACTGTGCACCATTGTA | TGACGTCGAAGTGACAGGTG | 81 |
| Sema3A | ATCAGTGGGTGCCTTACCAA | TCCGCCAAATGTTTTACTGG | 72 |
| neogenin | GGGGAATGAGACCAAAAATG | GGATGGGCACTAATCACAGG | 83 |
| BOC | GGACACCCATCCTGTACTACG | GAATGCCAGAAATGGTCCAG | 82 |
| CDO | GATTCCACAGAAGACACAGCAG | CCTCTGCTTCAGAATGACCAC | 94 |
| Unc5B | TTCCAGCTGCACACAACG | GCAGAGCAGAGAGCATCCA | 40 |
| Unc5C | GCACAGACCCCAGAATGAAT | CCCACGTAGAGAGCCACATC | 68 |
| embryonic MyHC | AGCCTCCAGCTTCAGAAGAA | GGCCCTCTCTGCCTCTATCT | 17 |
| perinatal MyHC | CCTGCAACTCCAGGTTCAAT | CTCTTTGATTTTGGCCTCCA | 73 |
| slow MyHC (I) | CAAGGTCAATACTCTGACCAAGG | CCATGCGCACCTTCTTCT | 78 |
| fast MyHC (IIa) | AGAGGGTTCTTGGCGAGAGT | AAGGCGCGGATGTTGTACT | 22 |
| fast MyHC (IIx) | TCTGCAGACGGAGTCAGGT | TTGAGTGAATGCCTGTTTGC | 33 |
| fast MyHC (IIb) | TGATGCAGGAGAAAAATGACC | ATCAGCTGGTCGCACCTTT | 1 |
| HPRT | CCTCCTCAGACCGCTTTTT | AACCTGGTTCATCATCGCTAA | 95 |