Table 3.
RT-qPCR primer sets for mouse netrin receptors and HPRT.
| Primer Name | Primer Sequence (5′ to 3′) Forward | Primer Sequence (5′ to 3′) Reverse | Amplicon (bp) |
|---|---|---|---|
| neogenin | TGAATTGTGAAGTTAATGCAGATTTGGTCC | TGTCCATGGATTCGTGAGCATATATGTTAG | 561 |
| BOC | GCCGAAGCTAATATCCCAGAAAGACTATAT | ACAGAGATATGTTGCCACCGATTATTGATT | 509 |
| CDO | GGAAGGCTTTTCATACAAACGTGTGAAATA | CAAGTCAAGATGTAAGGCTACAAACTACCT | 501 |
| DCC | GTATCTACGGCTTGAAACCAGCTGAATATA | CACATAGTTCCTCGAAAGTACTTCCAGAAA | 533 |
| Unc5A | GAGTCGCCCTCTCATCTCTACTACTG | GTTAAAGTTGATGTTGAAGCTCTGTCCATC | 519 |
| Unc5B | AACTAAGTAGACAGCTGTATACCCACTCTT | CACAATCAACTTGTCTCTACTCGATTCTCA | 552 |
| Unc5C | CTATTCAGAGATATGTGCTGGTGTAAGTCC | GAGCTTAAGTGTCAGCTTTTTAGAAACAGG | 563 |
| Unc5D | TCAAGAAGGGAGATAGACCTCAAACTAGTT | CTTCCTTGGAGACATTTATTCACTCACTCA | 592 |
| HPRT | CCGAGGATTTGGAAAAAGTGTTTATTCCTC | CTTTTCCAGTTTCACTAATGACACAAACGT | 555 |