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Optimization of media. (a) Carbon sources including glucose, fructose, sucrose, glycerol, crude glycerol, and N-acetyl glucosamine. (b) Nitrogen sources including yeast extract, malt extract, ammonium chloride, ammonium sulfate, peptone, and tryptone (0.1%). (c) Fructose concentration from 1% to 6%. (d) Yeast extract concentration from 0.05% to 1%. (e) Inoculum size from 0.5% to 4%. (f) Cultivation time from 24 to 168 h. DCW, dry cell weight.
