TABLE 1.
Customized TaqMan assays (primers/probes, Thermo Scientific Co, USA), Genbank accession numbers, and mean cycle threshold (Ct) value of the target genes utilized in this study
| Genes | Accession number | Customized assay |
Mean Ct cDNA 10 μg |
|---|---|---|---|
| Angiotensin‐converting enzyme 1 (ACE) | NM_009598.2 | Mm00802048_m1 | 22.9 ± 0.9 |
| Bradykinin receptor B1 (Bdkrb1) | NM_007539.2 | Mm04207315_s1 | 31.6 ± 0.4 |
| Bradykinin receptor B2 (Bdkrb2) | NM_009747.2 | Mm00437788_s1 | 30.6 ± 0.4 |
| Carboxypeptidase M (CPM) | NM_027468.1 | Mm01250796_m1 | 26.1 ± 0.3 |
| Vascular endothelial growth factor A (VEGF‐A) | NM_001025250.3 | Mm01281449_m1 | 22.5 ± 0.5 |
| Vascular endothelial growth factor receptor VEGF‐R2 (kinase insert domain protein receptor – Kdr) | NM_010612.2 | Mm01222421_m1 | 20.8 ± 0.4 |
| Hypoxia inducible factor (HIF−1) | NM_010431.2 | Mn00468869_m1 | 22.4 ± 0.5 |
| Beta‐actin | NM_007393.3 | Mm02619580_g1 | 19.4 ± 1.2 |
| Glyceraldehyde 3‐phosphate dehydrogenase (GAPDH) | NM_008084.2 | Mm99999915_g1 | 14.6 ± 0.4 |
| Beta−2 microglobulin (B2 M) | NM_009735.3 | Mm00437762_m1 | 18.7 ± 0.7 |
Cycle threshold values are expressed as mean of two technical replicates ± SEM.