TABLE 3.
Expression values using GAPDH and B2 M endogenous genes
| Normoxia | IH−1 | IH−2 | IH−3 | IH−4 | IH−5 | |
|---|---|---|---|---|---|---|
| HIF−1 | ||||||
| GAPDH | 1.0 ± 0.05 | 2.4 ± 0.3 | 3.0 ± 0.4* | 2.6 ± 0.5* | 3.0 ± 0.6* | 2.3 ± 0.2* |
| B2 M | 0.95 ± 0.1 | 1.6 ± 0.2 | 2.5 ± 0.5* | 2.0 ± 0.6 | 2.6 ± 0.4* | 1.7 ± 0.2 |
| VEGF‐A | ||||||
| GAPDH | 1.0 ± 0.1 | 2.1 ± 0.4 | 2.0 ± 0.3 | 1.6 ± 0.2 | 2.6 ± 0.3* | 2.6 ± 0.1* |
| B2 M | 1.0 ± 0.1 | 1.7 ± 0.2 | 2.6 ± 0.3 | 2.5 ± 0.8 | 3.2 ± 0.5* | 2.1 ± 0.6 |
| VEGF‐R2 | ||||||
| GAPDH | 1.0 ± 0.2 | 0.9 ± 0.1 | 2.1 ± 0.3 | 3.2 ± 0.6 | 3.2 ± 0.7 | 2.9 ± 0.6* |
| B2 M | 1.0 ± 0.1 | 0.78 ± 0.1 | 1.3 ± 0.2 | 1.7 ± 0.4 | 1.9 ± 0.1 | 2.4 ± 0.7* |
| B2R | ||||||
| GAPDH | 1.0 ± 0.2 | 1.6 ± 0.2 | 1.3 ± 0.1 | 2.1 ± 0.4* | 1.2 ± 0.08 | 1.1 ± 0.1 |
| B2 M | 0.97 ± 0.1 | 1.7 ± 0.1 | 2.9 ± 0.3* | 2.5 ± 0.3* | 2.3 ± 0.3* | 1.4 ± 0.2 |
| ACE | ||||||
| GAPDH | 1.0 ± 0.1 | 1.3 ± 0.1 | 1.5 ± 0.1 | 2.2 ± 0.4* | 1.2 ± 0.1 | 0.75 ± 0.1§ |
| B2 M | 0.9 ± 0.1 | 1.0 ± 0.1 | 1.4 ± 0.1*# | 1.6 ± 0.1* | 1.2 ± 0.05 | 0.63 ± 0.1§ |
| CPM | ||||||
| GAPDH | 1.0 ± 0.1 | 0.86 ± 0.1 | 1.2 ± 0.1 | 1.1 ± 0.1 | 0.95 ± 0.04 | 0.83 ± 0.07 |
| B2 M | 1.1 ± 0.1 | 0.94 ± 0.2 | 1.7 ± 0.2*# | 1.4 ± 0.2 | 1.6 ± 0.2 | 1.3 ± 0.2 |
HIF‐1, GAPDH: F (5,35) = 2.5782, p = 0.04, *different from normoxia (p = 0.04). B2 M: F (5,30) = 36,215, p = 0.011, *different from normoxia (p = 0.014). VEGF, GAPDH: F (5,32) = 26,461, p = 0.04, *different from normoxia (p = 0.04). B2 M: F (5,33) = 26,461, p = 0.04, *different from normoxia (p = 0.04). VEGF‐R2, GAPDH: F (5, 29) = 40,055, p = 0.001, *different from normoxia (p = 0.03). B2 M: F (5, 33) =26,461, p = 0.05. *different from normoxia (p = 0.04) and IH‐1 (p‐0.04). B2R, GAPDH: F (5, 37) = 30,874, p = 0.02, *different from normoxia and IH‐5 (p = 0.035). B2 M: F (5, 32) = 39,749, p = 0.006 *different from normoxia (p = 0.013); ACE, GAPDH: F (5, 37) =59,314, p < 0.001. *different from normoxia (p = 0.011), IH‐1 (p = 0.03), IH‐4 (p = 0.015), and IH‐5 (p = 0.0001). B2 M: F (5, 28) = 12,436, p = 0.0001. *different from normoxia (p = 0.01) and § different from IH‐1 (p = 0.02); # different from IH‐2 (p = 0.001) and IH‐3 (p = 0.001). CPM, GAPDH: F (5, 37) = 22,289, p = 0.07. B2 M: F (5, 33) = 24,485, p = 0.05. *different from normoxia (p = 0.04); §different from IH‐1 (p = 0.03).