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. 2021 Apr 26;22(9):4517. doi: 10.3390/ijms22094517

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© 2021 by the authors.

Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).

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Experimental protocol. Con = Control; Lev = Levosimendan, KHB = Krebs-Henseleit-Buffer; Vehicle = Krebs-Henseleit Buffer (KHB). Green bar: Hearts were perfused with a total of 22 mmol/L glucose concentration by combining 11 mmol/L glucose solution with KHB (containing 11 mmol/L glucose). Control (Con): Hearts were perfused with Krebs-Henseleit-Buffer (KHB) as vehicle for 10 min. Levosimendan 0.3 μM (Lev0.3): Hearts were perfused with 0.3 μM Lev for 10 min. Levosimendan 1 μM (Lev1): Hearts were perfused with 1 μM Lev for 10 min. Levosimendan 3 μM (Lev3): Hearts were perfused with 3 μM Lev for 10 min. Levosimendan 10 μM (Lev10): Hearts were perfused with 10 μM Lev for 10 min.