Figure 3.

Live/dead viability/cytotoxicity assay showing representative images of (A) DPSCs treated with Day 14 conditioned media obtained from 0.5% w/v NapFFεKεK-OH hydrogels, (B) vehicle control (α-MEM incubated for 14 days at 37 °C prior to cell treatment) and (C) DPSCs treated with 5% v/v Triton-X. Images A and B show mainly live cells, stained green with calcein AM, with very few cells stained red, whereas in C dead cells are stained red with EthD-1. Images were captured using an Evos FL Auto Imaging System. Scale bars: 250 µm.