Figure 5.

Effects of CHIR-090P, an LpxC inhibitor, on SDCL. BW25113 cells harboring pBAD24 (a), pBAD24-sulA (b) (SulA: 18.8 kDa) and pBAD24-soxS (c); (SoxS: 12.9 kDa) were grown in LB medium containing ampicillin. L-Arabinose (at a final concentration of 0.01%) and CHIR-090 (at a final concentration of 0, 1, 10, 100 or 1000 ng/mL) were added at OD₆₀₀ of about 0.5, and portions of the culture were taken at 24 h after induction. Sample preparation for SDS-PAGE was performed as described in the Materials and Methods Section.