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. 2021 Apr 27;22(9):4554. doi: 10.3390/ijms22094554

Figure 9.

Figure 9

CrASRs sequence alignment and GAL4 DNA binding domain-CrASRs fusion analyses for transactivation activity in yeast. (A) Sequence alignment of CrASR1, CrASR2, and CrASR3. The N-terminal His-rich motif, possible transcription activation domain, ABA/WDS domain, and C-terminal nuclear localization sequence were marked with black bold lines. (B) The GAL4 DNA binding domain was fused with 3 CrASRs and transformed into the yeast strain AH109 containing the His3 and LacZ reporter genes. Analysis of β-galactosidase activity of the relative yeast strains on plates. The yeast culture (OD600 to 2) was serially diluted to OD600 values of 0.2, 0.02 and 0.002, and then the 2-μL yeast liquid was spotted onto SD plates and cultured for 2 days at 30 °C.